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KMID : 0903519790220040210
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1979 Volume.22 No. 4 p.210 ~ p.216
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Studies on the Production of 5´ - Nucleotides by Streptomyces spp .
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김홍집/Kim, Hong Jip
배종찬/황규인/공운영/Bae, Chong Chan/Hwang, Kyu In/Kong, Un Young
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Abstract
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RNA degrading bacteria were isolated from soil of Korea. One strain (no. JSC-114), having strong 5´-phosphodiesterase activity, was identified as belonging to the genus Streptomyces on the basis of taxonomic characteristics.
The optimum conditions of 5´-phophosdiesterase production were found at 30℃ for 4 day in a medium containing 4.5% of soluble starch, 0.15% of peptone, 0.6% of yeast extract, 0.1% of MgSO₄·7H₂O, 0.01% of CaCl₂·2H₂O, 0.25% of KNO₃, and 0.5% of KH₂PO₄(pH 7.0).
The maximum production rate of 5´-nucleotides from yeast RVA was 95% at 40-45℃ for 4hrs, and the products were identified as 5´-IMP, 5´-GMP, 5´-CMP and 5´-UMP(5.5 : 5.0 : 4.9 : 5.0).
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